Optimization of Viable Glioblastoma Cryopreservation for Establishment of Primary Tumor Cell Cultures

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Background: The technology related to the establishment of primary tumor cell cultures of solid tumors, including glioblastoma, which is increasingly important for oncology research and practice. However, the processing of fresh tumor specimens for the formation of primary culture on the day of collection logistically difficult surgery. Feasibility cryopreservation of decent specimens of glioblastoma, allowing for major cultural establishments weeks to months after surgical tumor collection and freezing, shown by Mullins et al. in 2013, with a success rate of 59% which is not significantly lower than that achieved with fresh tumor tissue. However, research is targeting the optimization of glioblastoma cryopreservation protocol for the establishment of a culture worthy of the primary tumor has been limited.

Objective: The objective of this study was to optimize the cryopreservation method for cryobanking decent glioblastoma and to determine whether the two-dimensional (2D) or three-dimensional (3D) culture conditions that favor the growth of glioblastoma after thawing frozen tumor specimens. Methods: Part of eight human glioblastoma specimens cryopreserved by four different protocols differ in enzymatic digestion time (before or after cryopreservation), and in the cryopreservation media types (CryoStor CS10 or 10% dimethyl sulfoxide and 90% fetal calf serum).

After 1 month, the frozen tissue is thawed, enzymatically digested, if not digested in advance, and are used to culture primary tumor initiation 2D or 3D to determine eligibility. Results: Among the cryopreservation were tested and culture protocol, a combination of the most efficient of the cryopreservation and culture associated with the use of CryoStor CS10 cryopreservation medium, enzymatic digestion before it freezes, and 2D culturing after thawing the cultural level of success than 8 out of 8 cases (100% ). two-dimensional culture is generally more efficient to support the growth of tumor cells after thawing of 3D culture. Conclusion: The development of supporting worthy glioblastoma study cryopreservation methods based evidence for use in glioblastoma biobanking and research.

Optimization of Viable Glioblastoma Cryopreservation for Establishment of Primary Tumor Cell Cultures
Optimization of Viable Glioblastoma Cryopreservation for Establishment of Primary Tumor Cell Cultures

[Construction of RNA interference (RNAi) gene DEK lentiviral expression vector and its effect on the biological behavior of liver cancer cells]

Objective: To construct RNA interference (RNAi) gene DEK lentiviral expression vector, and to explore its effect on biological behavior of liver cancer cells. Methods: Double-stranded DNA oligo amplified and synthesized according to the sequence of the gene disruption DEK by RNAi technology. Disturb small RNA expression vector cloned pLKO.1 after enzymatic digestion. Recombinant lentiviral-sh pLKO.1 hDEK built, and then the virus supernatant was collected, packaged and infected by 293T cells. Real-time reverse transcription PCR (RT-PCR) and Western blot is used to detect the expression of DEK on human liver cancer cells Bel-7402, Hu-7, SmMC-7721 and HepG2, and DEK efficiency knockdown in each group of lentivirus-infected cells.

the ability of cell proliferation, cloning capabilities, apoptosis and migration ability was detected by cell counting kit-8 (CCK8), and the initial test flow cytometry, respectively. T-test was used to compare the mean between two groups, and one-way ANOVA was used to compare the groups. Results: enzymatic digestion and DNA sequencing results confirm that recombinant lentiviral vector pLKO.1-sh-sh hDEK1 and pLKO.1 hDEK3 successfully constructed. RT-PCR and Western blot results showed that DEK expression in human liver cancer cells BEL-7402 and higher Huh7 cells, and pLKO.1-sh hDEK3 more effective in inhibiting the DEK gene expression (P <0.05).

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh
PBMC-F2B - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Fresh
PBMC-F300M - Ask for price

Normal Human Peripheral Blood Mononuclear Cells, Frozen -100 million cells
79059-2 100 million cells
EUR 580
Description: Cryopreserved vial (30 x 10^6 cells) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from leukapheresis / apheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated for viability by flow cytometry. Cells were cryopreserved in serum-free Cryostor CS10 at a controlled rate.

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved
PBMC-C100M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved
PBMC-C10M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved
PBMC-C20M - Ask for price

Human Normal Peripheral Blood Mononuclear Cells (PBMC), Cryopreserved
PBMC-C50M - Ask for price

Human Peripheral Blood Mononuclear Cell Isolation and Viability Kit
K541-30 each
EUR 451.2

AnaPrep Blood DNA Extraction Kit 1200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 1 ml
Z1322002 1 kit (48 extractions) Including all required plastic disposables
EUR 419

AnaPrep Blood DNA Extraction Kit 200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 400 µl
Z1322001 1 kit (48 extractions) Including all required plastic disposables
EUR 227

Human Peripheral Blood CD3+ T Cells, Fresh
PBCD3-F20M - Ask for price

Human Peripheral Blood CD3+ T Cells, Cryopreserved
PBCD3-C10M - Ask for price

Human Peripheral Blood CD3+ T Cells, Cryopreserved
PBCD3-C20M - Ask for price

Immortalized Human Bone Marrow Mononuclear Cells
T0526 1x106 cells / 1.0 ml Ask for price

Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Fresh
PBCD4-25-F2M - Ask for price

Positively Selected Normal Peripheral Blood CD19+ B Cells, Fresh
PBCD19P-F10M - Ask for price

Human Normal Peripheral Blood CD4+/CD25+ Regulatory T Cells (T reg), Cryopreserved
PBCD4-25-C2M - Ask for price

Positively Selected Normal Peripheral Blood CD19+ B Cells, Cryopreserved
PBCD19P-C10M - Ask for price

Human Peripheral blood leukocyte (PBL's, WBC) lysate
HCL-1371 100ug
EUR 285.6

Human  Peripheral  Blood  CD14+ Monocytes, Fresh / Untouched
PBCD14U-F10M - Ask for price

Peripheral Blood Leukocyte Membrane Lysate
XBL-10813 0.1 mg
EUR 884.4
Description: Human peripheral blood leukocyte tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human peripheral blood leukocyte tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated peripheral blood leukocyte tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated peripheral blood leukocyte tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

cDNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte
C1234148-10 10 reactions
EUR 215

Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Untouched
PBCD14U-C10M - Ask for price

Human  Peripheral  Blood  CD14+ Monocytes, Fresh / Positive selection
PBCD14P-F10M - Ask for price

THP-1 Cell Slide (Human (1 yr infant, Male) peripheral blood monocyte, acute monocytic leukemia) (5 slides/pk)
HCLS-17008 1 pk
EUR 300

Total RNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte
R1234148-10 10 ug
EUR 183

Human  Peripheral  Blood  CD14+ Monocytes, Cryopreserved / Positive selection
PBCD14P-C10M - Ask for price

Total Protein - Human Adult Normal Tissue: Peripheral Blood Leukocyte
P1234148 1 mg
EUR 351

Human  Peripheral  Blood  CD4+  T  Lymphocytes, Fresh
PBCD4-F10M - Ask for price

Human  Peripheral  Blood  CD4+  T  Lymphocytes, Fresh
PBCD4-F20M - Ask for price

Membrane Protein - Human Adult Normal Tissue: Peripheral Blood Leukocyte
P3234148 0.1 mg
EUR 535

96 Well Human Adult Normal Genomic DNA Plate - Peripheral Blood Leukocyte
D8234148-1 1 plate
EUR 254

Human  Peripheral  Blood  CD4+  T  Lymphocytes, Cryopreserved
PBCD4-C10M - Ask for price

Methylated & Low-Methylated DNA Matched Pair - Human Peripheral Blood Leukocyte
D6234148-PP 2x5 ug
EUR 351

Genomic DNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte, from a single donor
D1234148 100 ug
EUR 286

Rat Anti Rat Rt6.1 (Peripheral T Cells) Monoclonal Antibody
CABT-49825RR 2 ml
EUR 639.6

HL-60 Cell Slide (Human (36yrs, Female) peripheral blood promyeloblast, acute promyelocytic leukemia) (5 slides/pk)
HCLS-17009 1 pk
EUR 300

Total RNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte, from 4 different donors, 2 male and 2 female. 1 ug/ul concentration
R1234148-10-P04 10x10 µg
EUR 2199

Peripheral Myelin Protein 22 (PMP22) Monoclonal Antibody (Human)
4-MAC746Hu21
  • EUR 306.00
  • EUR 3170.40
  • EUR 786.00
  • EUR 386.40
  • EUR 260.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Peripheral Myelin Protein 22 (PMP22)

Peripheral Myelin Protein 22 (PMP22) Monoclonal Antibody (Human), PE
4-MAC746Hu21-PE
  • EUR 367.20
  • EUR 3340.80
  • EUR 943.20
  • EUR 463.20
  • EUR 238.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with PE.

Peripheral Myelin Protein 22 (PMP22) Monoclonal Antibody (Human), APC
4-MAC746Hu21-APC
  • EUR 429.60
  • EUR 4146.00
  • EUR 1148.40
  • EUR 549.60
  • EUR 268.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with APC.

Peripheral Myelin Protein 22 (PMP22) Monoclonal Antibody (Human), Cy3
4-MAC746Hu21-Cy3
  • EUR 522.00
  • EUR 5478.00
  • EUR 1482.00
  • EUR 682.80
  • EUR 309.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with Cy3.

Peripheral Myelin Protein 22 (PMP22) Monoclonal Antibody (Human), HRP
4-MAC746Hu21-HRP
  • EUR 392.40
  • EUR 3613.20
  • EUR 1015.20
  • EUR 495.60
  • EUR 253.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with HRP.

Peripheral Myelin Protein 22 (PMP22) Monoclonal Antibody (Human), FITC
4-MAC746Hu21-FITC
  • EUR 367.20
  • EUR 3340.80
  • EUR 943.20
  • EUR 463.20
  • EUR 238.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with FITC.

Recombinant Human Peripheral Myelin Protein-2
7-05908 5µg Ask for price

Recombinant Human Peripheral Myelin Protein-2
7-05909 25µg Ask for price

Recombinant Human Peripheral Myelin Protein-2
7-05910 1mg Ask for price

Human Peripheral Myelin Protein 22 (PMP22) Protein
20-abx068468
  • EUR 760.80
  • EUR 326.40
  • EUR 2281.20
  • EUR 895.20
  • EUR 543.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Monoclonal Antibody (Human), APC-Cy7
4-MAC746Hu21-APC-Cy7
  • EUR 715.20
  • EUR 8148.00
  • EUR 2154.00
  • EUR 955.20
  • EUR 394.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with APC-Cy7.

ELISA kit for Human Peripheral myelin protein 22
EK3089 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Peripheral myelin protein 22 in samples from serum, plasma, tissue homogenates and other biological fluids.

Human Peripheral Myelin Protein 22 (PMP22) CLIA Kit
20-abx493870
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
DLR-PMP22-Hu-48T 48T
EUR 620.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Peripheral Myelin Protein 22 (PMP22) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
DLR-PMP22-Hu-96T 96T
EUR 807.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Peripheral Myelin Protein 22 (PMP22) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Peripheral myelin protein 22, PMP22 ELISA KIT
ELI-36130h 96 Tests
EUR 988.8

Human PMP22/ Peripheral myelin protein 22 ELISA Kit
E1979Hu 1 Kit
EUR 726

Human Peripheral myelin protein 22 (PMP22) ELISA Kit
abx250719-96tests 96 tests
EUR 895.2

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
20-abx152793
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Peripheral myelin protein 22 (PMP22) ELISA Kit
abx571655-96tests 96 tests
EUR 801.6

Human PMP22(Peripheral myelin protein 22) ELISA Kit
EH1441 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
SEC746Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Peripheral Myelin Protein 22 (PMP22) in Tissue homogenates, cell lysates and other biological fluids.

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
SEC746Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Peripheral Myelin Protein 22 (PMP22) in Tissue homogenates, cell lysates and other biological fluids.

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
SEC746Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Peripheral Myelin Protein 22 (PMP22) in Tissue homogenates, cell lysates and other biological fluids.

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
SEC746Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Peripheral Myelin Protein 22 (PMP22) in Tissue homogenates, cell lysates and other biological fluids.

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
4-SEC746Hu
  • EUR 5738.40
  • EUR 3031.20
  • EUR 768.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Peripheral Myelin Protein 22 (PMP22) in samples from Tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RD-PMP22-Hu-48Tests 48 Tests
EUR 625.2

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RD-PMP22-Hu-96Tests 96 Tests
EUR 867.6

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RDR-PMP22-Hu-48Tests 48 Tests
EUR 652.8

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RDR-PMP22-Hu-96Tests 96 Tests
EUR 907.2

Human Peripheral myelin protein 22(PMP22)ELISA Kit
YLA4457HU-48T 48T
EUR 435

Human Peripheral myelin protein 22(PMP22)ELISA Kit
YLA4457HU-96T 96T
EUR 562.5

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RD-PMP22-Hu-48T 48T
EUR 452.7
Description: tissue homogenates, cell lysates and other biological fluids.

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RD-PMP22-Hu-96T 96T
EUR 646.7
Description: tissue homogenates, cell lysates and other biological fluids.

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RDR-PMP22-Hu-48T 48T
EUR 475.34
Description: tissue homogenates, cell lysates and other biological fluids.

Human Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RDR-PMP22-Hu-96T 96T
EUR 679.04
Description: tissue homogenates, cell lysates and other biological fluids.

Peripheral Myelin Protein 22 (PMP22) Monoclonal Antibody (Human), Biotinylated
4-MAC746Hu21-Biotin
  • EUR 384.00
  • EUR 3110.40
  • EUR 912.00
  • EUR 472.80
  • EUR 267.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with Biotin.

Peripheral nerve tumor tissue array
SO1001a each
EUR 306
Description: Peripheral nerve tumor tissue array, including TNM and clinical stage, 50 cases/100 cores, replaced by SO1001b

Peripheral nerve tumor tissue array
SO1001b each
EUR 306
Description: Peripheral nerve tumor tissue array, including TNM and clinical stage, 50 cases/100 cores, replacing SO1001a

ELISA kit for Human PMP22 (Peripheral Myelin Protein 22)
ELK4471 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Peripheral Myelin Protein 22 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Recombinant Human Peripheral Myelin Protein-2, His Tag
7-05911 5µg Ask for price

Recombinant Human Peripheral Myelin Protein-2, His Tag
7-05912 25µg Ask for price

Recombinant Human Peripheral Myelin Protein-2, His Tag
7-05913 1mg Ask for price

Rat Peripheral myelin protein 22 (Pmp22)
1-CSB-CF018241RA
  • EUR 1449.60
  • EUR 578.40
  • EUR 885.60
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Rat Peripheral myelin protein 22(Pmp22) expressed in in vitro E.coli expression system

Peripheral Myelin Protein 22 (PMP22) Blocking Peptide
20-abx061188
  • EUR 343.20
  • EUR 510.00
  • 1 mg
  • 5 mg

PMP2 Peripheral Myelin Protein-2 Human Recombinant Protein
PROTP02689 Regular: 25ug
EUR 380.4
Description: PMP2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 14.9 kDa.

Peripheral Myelin Protein 22 (PMP22) Polyclonal Antibody (Human)
4-PAC746Hu01
  • EUR 296.40
  • EUR 3012.00
  • EUR 750.00
  • EUR 372.00
  • EUR 256.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Peripheral Myelin Protein 22 (PMP22)

Human Peripheral plasma membrane protein CASK, CASK ELISA KIT
ELI-33689h 96 Tests
EUR 988.8

Peripheral Myelin Protein 2 (PMP2) Antibody
20-abx114394
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx217831
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx328779
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx103057
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx173998
  • EUR 1095.60
  • EUR 560.40
  • 1 mg
  • 200 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx177954
  • EUR 1479.60
  • EUR 710.40
  • 1 mg
  • 200 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx177955
  • EUR 1479.60
  • EUR 710.40
  • 1 mg
  • 200 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx177956
  • EUR 543.60
  • EUR 159.60
  • EUR 1562.40
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx131854
  • EUR 410.40
  • EUR 1028.40
  • EUR 526.80
  • EUR 184.80
  • EUR 309.60
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx133606
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Peripheral Myelin Protein 22 (PMP22) Antibody
20-abx304860
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Peripheral Myelin Protein 22 (PMP22) Antibody
abx028584-400ul 400 ul
EUR 627.6

Peripheral Myelin Protein 22 (PMP22) Antibody
abx028584-80l 80 µl
EUR 343.2

Recombinant rat Peripheral myelin protein 22
P1725 100ug Ask for price
Description: Recombinant protein for rat Peripheral myelin protein 22

Peripheral Myelin Protein 22 (PMP22) Polyclonal Antibody (Human), PE
4-PAC746Hu01-PE
  • EUR 355.20
  • EUR 3168.00
  • EUR 900.00
  • EUR 446.40
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with PE.

Peripheral Myelin Protein 22 (PMP22) Polyclonal Antibody (Human), APC
4-PAC746Hu01-APC
  • EUR 414.00
  • EUR 3930.00
  • EUR 1094.40
  • EUR 528.00
  • EUR 262.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with APC.

Peripheral Myelin Protein 22 (PMP22) Polyclonal Antibody (Human), Cy3
4-PAC746Hu01-Cy3
  • EUR 502.80
  • EUR 5190.00
  • EUR 1410.00
  • EUR 654.00
  • EUR 301.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with Cy3.

Peripheral Myelin Protein 22 (PMP22) Polyclonal Antibody (Human), HRP
4-PAC746Hu01-HRP
  • EUR 379.20
  • EUR 3426.00
  • EUR 968.40
  • EUR 477.60
  • EUR 247.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with HRP.

Recombinant mouse Peripheral myelin protein 22
P1724 100ug Ask for price
Description: Recombinant protein for mouse Peripheral myelin protein 22

Recombinant Peripheral Myelin Protein 22 (PMP22)
4-RPC746Hu01
  • EUR 539.33
  • EUR 267.60
  • EUR 1692.48
  • EUR 644.16
  • EUR 1168.32
  • EUR 436.80
  • EUR 4051.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Peripheral Myelin Protein 22 expressed in: E.coli

Peripheral Myelin Protein 22 (PMP22) Polyclonal Antibody (Human), FITC
4-PAC746Hu01-FITC
  • EUR 355.20
  • EUR 3168.00
  • EUR 900.00
  • EUR 446.40
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with FITC.

Rat Peripheral Myelin Protein 22 (PMP22) Protein
20-abx654683
  • EUR 777.60
  • EUR 326.40
  • EUR 2331.60
  • EUR 910.80
  • EUR 560.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

ELISA kit for Rat Peripheral myelin protein 22
EK3088 96 tests
EUR 804
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Peripheral myelin protein 22 in samples from serum, plasma, tissue homogenates and other biological fluids.

Neuroblastoma and peripheral nerve tissue array
NB642c each
EUR 306
Description: Neuroblastoma and peripheral nerve tissue array, with INSS stage, IHC marker (CD56/CgA) info, 32 cases/64 cores (core size 1.5mm), replacing NB642b

Peripheral Myelin Protein 22 (PMP22) Antibody (HRP)
20-abx304861
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Rat Peripheral Myelin Protein 22 (PMP22) CLIA Kit
20-abx493872
  • EUR 10282.80
  • EUR 5472.00
  • EUR 1262.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Peripheral Myelin Protein 22 (PMP22) Antibody (FITC)
20-abx304862
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Mouse Peripheral Myelin Protein 22 (PMP22) Protein
20-abx654682
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Peripheral Myelin Protein 22 (PMP22) Protein
20-abx654684
  • EUR 794.40
  • EUR 326.40
  • EUR 2448.00
  • EUR 944.40
  • EUR 577.20
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Normal Human Peripheral nerve (single section per slide) (5 slides/pack)
HuFPT146 5 slides
EUR 150

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
20-abx155988
  • EUR 8684.40
  • EUR 4626.00
  • EUR 1074.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Cow Peripheral myelin protein 22 (PMP22) ELISA Kit
abx516097-96tests 96 tests
EUR 1093.2

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
DLR-PMP22-Ra-48T 48T
EUR 658.8
Description: A sandwich quantitative ELISA assay kit for detection of Rat Peripheral Myelin Protein 22 (PMP22) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
DLR-PMP22-Ra-96T 96T
EUR 861.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat Peripheral Myelin Protein 22 (PMP22) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat Pmp22/ Peripheral myelin protein 22 ELISA Kit
E0773Ra 1 Kit
EUR 775.2

Rat Pmp22(Peripheral myelin protein 22) ELISA Kit
ER0487 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.094 ng/ml

Rat Peripheral myelin protein 22 (PMP22) ELISA Kit
abx256464-96tests 96 tests
EUR 895.2

Rat Peripheral myelin protein 22 (PMP22) ELISA Kit
abx571185-96tests 96 tests
EUR 886.8

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
SEC746Ra-10x96wellstestplate 10x96-wells test plate
EUR 6149.04
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Peripheral Myelin Protein 22 (PMP22) in tissue homogenates, cell lysates and other biological fluids.

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
SEC746Ra-1x48wellstestplate 1x48-wells test plate
EUR 611.57
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Peripheral Myelin Protein 22 (PMP22) in tissue homogenates, cell lysates and other biological fluids.

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
SEC746Ra-1x96wellstestplate 1x96-wells test plate
EUR 822.24
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Peripheral Myelin Protein 22 (PMP22) in tissue homogenates, cell lysates and other biological fluids.

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
SEC746Ra-5x96wellstestplate 5x96-wells test plate
EUR 3340.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Peripheral Myelin Protein 22 (PMP22) in tissue homogenates, cell lysates and other biological fluids.

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
4-SEC746Ra
  • EUR 6210.00
  • EUR 3280.80
  • EUR 823.20
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Peripheral Myelin Protein 22 (PMP22) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat Peripheral Myelin Protein 22(PMP22)ELISA kit
QY-E10198 96T
EUR 433.2

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RD-PMP22-Ra-48Tests 48 Tests
EUR 668.4

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RD-PMP22-Ra-96Tests 96 Tests
EUR 930

Rat Peripheral Myelin Protein 22 ELISA Kit (PMP22)
RK03897 96 Tests
EUR 625.2

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RDR-PMP22-Ra-48Tests 48 Tests
EUR 699.6

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RDR-PMP22-Ra-96Tests 96 Tests
EUR 973.2

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RD-PMP22-Ra-48T 48T
EUR 487.7
Description: tissue homogenates, cell lysates and other biological fluids.

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RD-PMP22-Ra-96T 96T
EUR 696.7
Description: tissue homogenates, cell lysates and other biological fluids.

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RDR-PMP22-Ra-48T 48T
EUR 512.09
Description: tissue homogenates, cell lysates and other biological fluids.

Rat Peripheral Myelin Protein 22 (PMP22) ELISA Kit
RDR-PMP22-Ra-96T 96T
EUR 731.54
Description: tissue homogenates, cell lysates and other biological fluids.

PMP2 Human, Peripheral Myelin Protein-2 Human Recombinant Protein, His Tag
PROTP02689-1 Regular: 25ug
EUR 380.4
Description: PMP2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 19.41kDa. PMP2 is fused to His tag at N-terminus and purified by standard chromatography techniques.

MECA-79 Antibody / Peripheral Node Addressin / PNAd
V8344-100UG 100 ug
EUR 349.3
Description: MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 beta1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with Mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.

MECA-79 Antibody / Peripheral Node Addressin / PNAd
V8344-20UG 20 ug
EUR 153.3
Description: MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 beta1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with Mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.

MECA-79 Antibody / Peripheral Node Addressin / PNAd
V8344SAF-100UG 100 ug
EUR 349.3
Description: MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 beta1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with Mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.

Mouse Peripheral Myelin Protein 22 (PMP22) CLIA Kit
20-abx493871
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Peripheral Myelin Protein 22 (PMP22) Polyclonal Antibody (Human), APC-Cy7
4-PAC746Hu01-APC-Cy7
  • EUR 685.20
  • EUR 7716.00
  • EUR 2046.00
  • EUR 912.00
  • EUR 382.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Peripheral Myelin Protein 22 (PMP22). This antibody is labeled with APC-Cy7.

Peripheral Myelin Protein 22 (PMP22) Antibody (Biotin)
20-abx304863
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Mouse Peripheral Myelin Protein 22 (PMP22) ELISA Kit
20-abx154561
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Peripheral Myelin Protein 22 (PMP22) ELISA Kit
DLR-PMP22-Mu-48T 48T
EUR 632.4
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Peripheral Myelin Protein 22 (PMP22) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse Peripheral Myelin Protein 22 (PMP22) ELISA Kit
DLR-PMP22-Mu-96T 96T
EUR 825.6
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Peripheral Myelin Protein 22 (PMP22) in samples from tissue homogenates, cell lysates or other biological fluids.

Reactive Oxygen Species Induces Differentiation of endothelial Liver Cancer Stem-Like Cells Sphere through the activation of Akt / IKK Signaling Pathway

Cancer stem cells (CSC) of a variety of cancers can be transdifferentiate into endothelial cells and further form functional blood vessels, suggesting possible resistance mechanism other antiangiogenic agents. However, it remains unclear whether CSC of hepatocellular carcinoma has the ability to differentiate into endothelial cells, and the resulting resistance to antiangiogenic therapies targeting VEGF. Reactive oxygen species (ROS) are involved in self-renewal and differentiation of CSC, however, their role in endothelial differentiation of CSC has been poorly understood. In this study, we found that the cancer stem-like ball of cells enriched from cell line hepatocellular carcinoma human Hep G2 can differentiate into endothelial cells morphologic and functional, and this process could be blocked by Akt1 / 2 kinase inhibitor and IKK-β inhibitor BAY 11 -7082 but not with Bevacizumab, VEGFA-binding antibody, and dAPT, γ-Secretase inhibitor.

Both hydrogen peroxide and BSO (inhibitor of GSH biosynthesis) induce differentiation of cancer stem-like cells ball into endothelial cells, which can be canceled by the antioxidant N-Acetyl-L-cysteine ​​(NAC). We also found that hydrogen peroxide or BSO induces phosphorylation of Akt and IKK scope of differentiated endothelial cells.

Thus, both Akt1 / 2 kinase inhibitor BAY 11-7082 and inhibits hydrogen peroxide and BSO-mediated endothelial differentiation of cancer stem-like cells of the ball. Collectively, these results indicate that the cancer stem-like ball of Hep G2 cells can differentiate into endothelial cells both morphologically and functionally, and this process is independent of VEGF and NOTCH signaling but depends on the activation of Akt and IKK. ROS promotes endothelial differentiation of cancer stem-like cells through activation of Akt scope / IKK signaling pathways. Therefore, our study suggests a new mechanism of resistance to conventional antiangiogenic therapy and may provide potential therapeutic targets for the treatment of liver cancer.

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