Objective: Few studies have investigated small cell lung carcinoma (EPSCC) in a systematic way. This study is to analyze EPSCC at 11 sites in the tumors of various aspects of the United States (1975-2016). Methods: 4397 patients diagnosed with EPSCC in 11 primary tumor site is selected from the Surveillance, Epidemiology and End Results (SEER) database. EPSCC incidents in the last decade and the survival rate 1, 3 and 5 years respectively also roughly calculated the tumor site. Prognostic factors EPSCC performed with Cox regression analysis. Results: Statistically, EPSCC incidence is on the rise during the last 30 years. Of the 11 sites of the primary tumor, the bladder is most often affected while the stomach and kidneys are rarely affected. Men are more prone to EPSCC than women. patients who have been married more often attacked EPSCC, but had to survive longer. The most intensive cases in California and a rising trend has been observed. The overall level of 5-year survival (OS) ranged from 2.0% to 42.5% in patients with EPSCC in 11 tumor site (P <0.001). OS is better for EPSCC in the breast and cervix. However, citing tumors in the colon, esophagus, pancreas, rectum and stomach are all associated with poorer survival. Characteristics and prognosis of EPSCC in different tumor sites were statistically significant (P <0.001). Age, gender, marital status, stage, surgery, radiotherapy and chemotherapy is equally significant factor EPSCC patient survival (P <0.05). Conclusion: There is a tendency to increased incidence of EPSCC. EPSCC survival in different tumor sites were significantly different. In addition to tumor location, age, gender, marital status, stage, surgery, radiotherapy and chemotherapy are all important factors for survival. This study has implications for the prevention and treatment of EPSCC.

Produce primary murine fibroblast cell lines vagina

vulvovaginal human fibroblast cell lines are useful for studying the major underlying biological mechanisms of genital pain, pelvic organ prolapse, and the spread of sexually transmitted infections. However, vaginal biopsy is required to establish this cell line is invasive and relatively difficult to obtain. primary mouse fibroblast cell lines derived from pre-clinical animal models of this condition can be used for more controlled experiments that can help us to dissect the mechanism of the disease. To our knowledge, no published protocol for establishing primary murine fibroblast cell lines the vagina until today. Here, we describe a protocol for the establishment of the vagina murine fibroblast cell line by enzymatic digestion of the vaginal canal network. cell lines generated using this method can be used for in vitro studies of this important structural cells in various pre-clinical mouse model; The study is required to identify and characterize therapeutic targets and the relevant regulations in various diseases of interest. As indicated in our representation of data, this protocol resulted in viable cell lines from ND4 Swiss outbred mice. These cells bear surface markers characteristic of fibroblasts and is capable of producing inflammatory cytokines in response to treatment with bacteria and yeast antigens in vitro. Rumen epithelial inflammation may occur during ruminal acidosis as a result of translocation and interaction from the rumen epithelial cells (REC) with molecules such as lipopolysaccharide (LPS). inflammatory as it has been reported to modify cellular processes such as the absorption of nutrients, regulation of metabolism and energy substrate utilization in other types of cells, but not researched for REC

Neurotoxic potential of polystyrene nanoplastics in primary cells originating from mouse brain
Neurotoxic potential of polystyrene nanoplastics in primary cells originating from mouse brain

Analysis of the correlation between changes in the N-glycans and invasive in liver cancer cell lines

N-glycoforms of glycoproteins modify protein function and control a number of biological pathways. The purpose of this study was to determine the relationship between changes in the N-glycans and cancer aggressiveness in terms of the ability of the cancer cell invasion. The expression of urokinase-type plasminogen activator (uPA) and N-acetylglucosaminyltransferase V (GNT-V) in liver cancer cell lines were analyzed by western blotting. Invasive cells were analyzed by Matrigel invasion test. uPA and GNT-V expression in liver cancer cell lines was knocked down by RNA interference. Furthermore, uPA is expressed in liver cancer cells using lentiviral vectors, and HepG2 cells expressing mutant strain uPA deficiency of N-glycans established. An assisted glycoblotting matrix-assisted laser desorption / ionization-time-of-flight / mass spectrometry-based quantitative analysis of liver cancer cell lines do, where invasive amended by modifying the expression of uPA and GNT-V. N-glycan profile was found to differ between highly invasive liver cancer cell lines HLE and less invasive cell line HepG2. Expression of some N-glycans, including forms with m / z = 1892, amended in accordance with invasive controlled by the knockdown and overexpression of uPA. The invasion of HepG2 cells with mutant uPA did not increase regardless of the level of expression of uPA. Here GNT-V knockdown and alteration of N-glycan, uPA expression did not change, whereas decreased cell invasion.

Bovine Primary Lung Cells, fetal

ABC-TC5513 1 vial Ask for price
Description: The Bovine Fetal Primary Lung cells are isolated from lung tissue of a bovine fetus. It is often used as a model to study various bovine respiratory viruses including bovine parainfluenza virus, bovine viral diarrhea virus, and bovine herpesvirus 1 that greatly affect bovine health and related industry.

Bovine Primary Lung Cells, fetal

T0090 5x10^5 cells / 1.0 ml
EUR 625

Bovine Kidney Cells, Fetal

ABC-TC4288 1 vial Ask for price
Description: Source Organ: Kidney; Growth Properties: Adherent; Morphology: Polygonal; Disease: Normal

Normal Human Primary Kidney Podocytes

T5519 2x10^5 cells / 1.0 ml Ask for price

Immortalized Fetal Bovine Kidney Cells - SV40

T0081 1x106 cells / 1.0 ml Ask for price

Human 293, transformed primary embryonal kidney lysate

HCL-1210 100ug
EUR 255.6

Rat Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-82556 1 Kit Ask for price

Mouse Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-82057 1 Kit Ask for price

Human Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-96075 1 Kit Ask for price

Bovine Primary Lymphocytes

T4150 5x10^5 cells / 1.0 ml
EUR 975

Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Kidney

D8235142-PP-10 2x10 ug
EUR 416

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Kidney

C8235142-PP 10 reactions x2
EUR 530

Rat Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium

9-25056 5 x 100 ml Ask for price

Matched Pair - DNA - Human Primary and Metastatic Tumor Tissue: Kidney

D8235142-PM-10 2x10 ug
EUR 690

Mouse Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium

9-32057 5 x 100 ml Ask for price

Human Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium

9-46075 5 x 100 ml Ask for price

Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Kidney

R8235142-PP-10 2x10 ug
EUR 463

Human Cancer PrimaCell9: Kidney Tumor Cells

2-96032 1 Kit Ask for price

Matched Pair - Total RNA - Human Primary and Metastatic Tumor Tissue: Kidney

R8235142-PM-10 2x10 ug
EUR 716

Matched Pair - cDNA - Human Primary and Matched Metastatic Tumor Tissue: Kidney

C8235142-PM 10 reactions x2
EUR 1102

Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Kidney

P8235142-PP 0.2 mg x2
EUR 663

Rat Kidney PrimaCell 6: Proximal Tubular Cells

2-82594 1 Kit Ask for price

Matched Pair - Frozen Tissue Section - Human Primary Tumor and Normal (PP): Kidney

T6235142-PP 5 slides x2
EUR 583

Mouse Kidney PrimaCell 8: Proximal Tubular Cells

2-82099 1 Kit Ask for price

Human Kidney PrimaCell 8: Proximal Tubular Cells

2-96120 1 Kit Ask for price

Matched Pair - Paraffin Tissue Section - Human Primary Tumor and Normal (PP): Kidney

T8235142-PP 5 slides x2
EUR 375

Mouse Kidney PrimaCell6: Normal Renal Mesangial Cells

2-82061 1 Kit Ask for price

Human Kidney PrimaCell5: Normal Renal Mesangial Cells

2-96078 1 Kit Ask for price

Bovine Kidney cDNA

BD-901 30 reactions
EUR 243

BOVINE, KIDNEY, FRESH

8600832 1EA
EUR 45.13

BOVINE, KIDNEY, FROZEN

8620832 1EA
EUR 45.13

Bovine Primary hepatic carcinoma ELISA kit

E01A85584 96T
EUR 700
Description: ELISA

Human Primary Colon Cells

T4056 5x10^5 cells / 1.0 ml Ask for price

Rat Primary Schwann Cells

T4841 5x10^5 cells / 1.0 ml Ask for price

Matched Pair - Total Protein - Human Primary Tumor and Metastatic Tumor Tissue (PM): Kidney

P8235142-PM 0.2 mg x2
EUR 1325

Rat Kidney PrimaCell1: Normal Glomerular Endothelial Cells

2-82555 1 Kit Ask for price

Human Primary Schwann Cells

T4176 5x10^5 cells / 1.0 ml Ask for price

Rat Primary Mesangial Cells

T4932 5x10^5 cells / 1.0 ml Ask for price

Matched Pair - Frozen Tissue Section - Human Primary and Matched Metastatic Tumor (PM): Kidney

T6235142-PM 5 slides x2
EUR 736

Human Cancer PrimaCell9: Kidney Tumor Cells Growth Medium

9-46032 5 x 100 ml Ask for price

Calf Primary Thyrocyte Cells

T0400 5x10^5 cells / 1.0 ml
EUR 975

Mouse Kidney PrimaCell1: Normal Glomerular Endothelial Cells

2-82056 1 Kit Ask for price

Human Kidney PrimaCell1: Normal Glomerular Endothelial Cells

2-96074 1 Kit Ask for price

Human Primary Mesangial Cells

T4086 5x10^5 cells / 1.0 ml
EUR 975

Human Primary Dendritic Cells

T4021 5x10^5 cells / 1.0 ml
EUR 1375

Matched Pair - Paraffin Tissue Section - Human Primary and Matched Metastatic Tumor (PM): Kidney

T8235142-PM 5 slides x2
EUR 626

Rat Kidney Cells

ABC-TC4145 1 vial Ask for price
Description: Rat primary kidney cells (RPKIDCs) are isolated from tissue of 1-day-old neonatal Sprague–Dawley rats. RPKIDCs are mixed population of mouse kidney cells grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

Calf Primary Parathyroid Cells

T0410 5x10^5 cells / 1.0 ml
EUR 625

Human Primary Urothelial Cells

T4160 5x10^5 cells / 1.0 ml Ask for price

Human Primary Mesothelial Cells

T4067 5x10^5 cells / 1.0 ml
EUR 1375

Rat Kidney PrimaCell5: Normal Renal Artery Endothelial Cells

2-82559 1 Kit Ask for price

Rat Kidney PrimaCell8: Normal Renal Tubular Epithelial Cells

2-82562 1 Kit Ask for price

Bovine Kidney Total RNA

BR-901 0.1mg
EUR 160

Primary Rat Leydig Cells (LC)

CSI119Ra01 5X10^5
EUR 920

Mouse Kidney PrimaCell4: Normal Renal Artery Endothelial Cells

2-82059 1 Kit Ask for price

Mouse Kidney PrimaCell8: Normal Renal Tubular Epithelial Cells

2-82063 1 Kit Ask for price

Human Kidney PrimaCell3: Normal Renal Artery Endothelial Cells

2-96076 1 Kit Ask for price

Human Kidney PrimaCell7: Normal Renal Tubular Epithelial Cells

2-96080 1 Kit Ask for price

Primary Rat Schwann Cells  (SC)

CSI038Ra01 5X10^5
EUR 1180

Bovine Kidney Genomic DNA

BG-901 0.1mg
EUR 177

Human Primary CD3+ Blood Cells

T5401 5x10^5 cells / 1.0 ml
EUR 975

Primary Rat Sertoli Cells (SEC)

CSI112Ra01 5X10^5
EUR 960

Primary Mouse Leydig Cells (LC)

CSI119Mu01 5X10^5
EUR 780

Rat Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

9-26056 1 Set Ask for price

Human Primary CD34+ Blood Cells

T5402 5x10^5 cells / 1.0 ml
EUR 975

Primary Rat Meningeal Cells (MC)

CSI047Ra01 5X10^5
EUR 880

Primary Rat Microglia Cells (MC)

CSI049Ra01 5X10^5
EUR 1320

Primary Canine Leydig Cells (LC)

CSI119Ca01 5X10^5
EUR 1260

Primary Rabbit Leydig Cells (LC)

CSI119Rb01 5X10^5
EUR 960

Primary Rabbit Tendon Cells (TC)

CSI249Rb01 5X10^5
EUR 850

Bovine Kidney Tissue Lysate

IBOKDTL100UG each
EUR 541
Description: Bovine Kidney Tissue Lysate

Bovine Kidney Total Protein

BT-901 1mg
EUR 153

Mouse Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

9-33057 1 Set Ask for price

Human Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

9-47075 1 Set Ask for price

Mouse Primary Neural Stem Cells

T4173 5x10^5 cells / 1.0 ml Ask for price

Human Primary Neural Stem Cells

T4178 5x10^5 cells / 1.0 ml
EUR 1375

Primary Rat Myocardial Cells (MC)

CSI007Ra01 5X10^5
EUR 1300

Primary Mouse Sertoli Cells (SEC)

CSI112Mu01 5X10^5
EUR 980

Primary Canine Sertoli Cells (SEC)

CSI112Ca01 5X10^5
EUR 1280

Dog Primary CD3+ Pan T Cells

T5495 1x10^6 cells / 1.0 ml
EUR 1425

Dog Primary CD3+ Pan T Cells

T5496 1x10^6 cells / 1.0 ml
EUR 1425

Dog Primary CD3+ Pan T Cells

T5497 1x10^6 cells / 1.0 ml
EUR 1425

Bovine KIDNEY WHOLE 2 EA*

57120-2 2 EA
EUR 209.81

Bovine Kidney Frozen Sections

BF-901 10 slides
EUR 261

Rat Primary Lung Epithelial Cells

T4800 5x10^5 cells / 1.0 ml
EUR 625

Rat Primary Skeletal Muscle Cells

T4834 5x10^5 cells / 1.0 ml Ask for price

Primary Mouse Myocardial Cells (MC)

CSI007Mu01 5X10^5
EUR 1300

Primary Rabbit Meningeal Cells (MC)

CSI047Rb01 5X10^5
EUR 1280

Primary Rabbit Synovial Cells (SYC)

CSI114Rb01 5X10^5
EUR 1150

Rat Primary Renal Epithelial Cells

T4936 5x10^5 cells / 1.0 ml Ask for price

Primary Canine Myocardial Cells (MC)

CSI007Ca01 5X10^5
EUR 3500

Primary Rabbit Myocardial Cells (MC)

CSI007Rb01 5X10^5
EUR 1400

Bovine Kidney Paraffin Sections

BP-901 10 slides
EUR 240

Mouse Kidney PrimaCell6: Normal Renal Mesangial Cells Growth Medium

9-32061 5 x 100 ml Ask for price

Human Kidney PrimaCell5: Normal Renal Mesangial Cells Growth Medium

9-46078 5 x 100 ml Ask for price

Human Primary Skeletal Muscle Cells

T4069 5x10^5 cells / 1.0 ml
EUR 625

Human Primary Fungiform Taste Cells

T4105 5x10^5 cells / 1.0 ml
EUR 625

Mouse Primary Skeletal Muscle Cells

T4990 5x10^5 cells / 1.0 ml
EUR 975

Human Primary Prostate Stromal Cells

T4080 5x10^5 cells / 1.0 ml Ask for price

Human Primary Renal Epithelial Cells

T4090 5x10^5 cells / 1.0 ml Ask for price

Human Primary Nasal Epithelial Cells

T4014 5x10^5 cells / 1.0 ml
EUR 1375

Rat Primary Bladder Epithelial Cells

T4940 5x10^5 cells / 1.0 ml Ask for price

Rat Primary Dermal Endothelial cells

T4964 5x10^5 cells / 1.0 ml Ask for price

Primary Rat Spermatogonium Cells (SPC)

CSI245Ra01 5X10^5
EUR 780

Human Primary Liver Mononuclear Cells

T4222 5x10^5 cells / 1.0 ml Ask for price

Rat Primary Tracheal Epithelial Cells

T4804 5x10^5 cells / 1.0 ml Ask for price

Rat Primary Cervical Epithelial Cells

T4952 5x10^5 cells / 1.0 ml Ask for price

Rat Primary Prostate Epithelial Cells

T4956 5x10^5 cells / 1.0 ml Ask for price

Human Primary Thymic Epithelial Cells

T4980 5x10^5 cells / 1.0 ml
EUR 975

Human Primary Corneal Epithelial Cells

T4058 5x10^5 cells / 1.0 ml
EUR 1475

Human Primary Oral Keratinocytes Cells

T4074 5x10^5 cells / 1.0 ml Ask for price

Human Primary Mammary Epithelial Cells

T4036 5x10^5 cells / 1.0 ml
EUR 625

Human Primary Aortic Endothelial Cells

T4039 5x10^5 cells / 1.0 ml
EUR 625

Human Primary Bladder Epithelial Cells

T4161 5x10^5 cells / 1.0 ml Ask for price

Human Primary Tonsil Endothelial Cells

T4191 5x10^5 cells / 1.0 ml Ask for price

Mouse Primary Aortic Endothelial Cells

T4592 5x10^5 cells / 1.0 ml Ask for price

Mouse Primary Bladder Epithelial Cells

T4640 5x10^5 cells / 1.0 ml
EUR 975

Mouse Primary Nephron Progenitor Cells

T4644 5x10^5 cells / 1.0 ml Ask for price

Rat Primary Bronchial Epithelial Cells

T4803 5x10^5 cells / 1.0 ml Ask for price

Mouse Primary Corneal Epithelial Cells

T4571 5x10^5 cells / 1.0 ml
EUR 1375

Human Primary Prostate Epithelial Cells

T4078 5x10^5 cells / 1.0 ml
EUR 625

Human Primary Tracheal Epithelial Cells

T4007 5x10^5 cells / 1.0 ml
EUR 625

Human Primary Amniotic Epithelial Cells

T4109 5x10^5 cells / 1.0 ml
EUR 625

Human Primary Trabecular Meshwork Cells

T4182 5x10^5 cells / 1.0 ml
EUR 975

Human Primary Pancreatic Stellate Cells

T4215 5x10^5 cells / 1.0 ml
EUR 975

Mouse Primary Tracheal Epithelial Cells

T4502 5x10^5 cells / 1.0 ml Ask for price

Mouse Primary Cervical Epithelial Cells

T4650 5x10^5 cells / 1.0 ml Ask for price

Mouse Primary Prostate Epithelial Cells

T4654 5x10^5 cells / 1.0 ml Ask for price

Rat Primary Esophageal Epithelial Cells

T4912 5x10^5 cells / 1.0 ml Ask for price

Primary Canine Spermatogonium Cells (SPC)

CSI245Ca01 5X10^5
EUR 960

Rat Kidney PrimaCell1: Normal Glomerular Endothelial Cells Growth Medium

9-25055 5 x 100 ml Ask for price

Human Primary Placental Epithelial Cells

T4076 5x10^5 cells / 1.0 ml
EUR 625

Human Primary Bronchial Epithelial Cells

T4006 5x10^5 cells / 1.0 ml
EUR 625

Primary Rat peritoneal mesothelial cells

CSI0248Ra01 5X10^5
EUR 860

Human Primary Esophageal Epithelial Cells

T4230 5x10^5 cells / 1.0 ml
EUR 1375

Mouse Primary Esophageal Epithelial Cells

T4612 5x10^5 cells / 1.0 ml Ask for price

Mouse Kidney PrimaCell1: Normal Glomerular Endothelial Cells Growth Medium

9-32056 5 x 100 ml Ask for price

Human Kidney PrimaCell1: Normal Glomerular Endothelial Cells Growth Medium

9-46074 5 x 100 ml Ask for price

Human Kidney Cells, Uncultured

ABC-TC3664 1 vial Ask for price
Description: Human whole kidney cells are derived from whole kidneys that have been dissociated into single cells and frozen. Human whole kidney cells are from a single donor. These cells enable researchers to produce various cell types such as glomerular, cortical, and tubular epithelial cells found within the urinary system using standard published protocols. Human whole kidney cells may be used for various types of in vitro studies, drug screening toxicity assays, viral studies, or transplantation studies into normal or diseased systemsDevelopment period: Prenatal

Bovine Membrane primary amine oxidase, AOC3 ELISA KIT

ELI-03531b 96 Tests
EUR 1113.6

Bovine Membrane primary amine oxidase (AOC3) ELISA Kit

RK15022 96T
EUR 280

Recombinant Bovine Primary amine oxidase, lung isozyme,partial

CSB-EP524305BO 10926 mg Ask for price

Human Primary Fetal Liver-CD34+ Cells

T4066 3x10^5 cells / 1.0 ml Ask for price

Human Primary Alveolar Cells-Type 1

T4003 5x10^5 cells / 1.0 ml Ask for price

Rat Primary Aortic Smooth Muscle Cells

T4820 5x10^5 cells / 1.0 ml Ask for price

One N-glycan (m / z = 1892) is common among the N-glycans in the comparative analysis between HLE and HepG2, HLE and uPA knockdown HLE, HepG2 and uPA-expressing HepG2 and HLE and GNT-V cells knockdown HLE and in among the N-glycan profile in human uPA. Therefore, N-glycosylation is an important factor controlling liver cancer cell invasion, and a special N-glycan (m / z = 1892) associated with liver cancer cell invasion through uPA identified in this study.