Viromer® CRISPR

for RNP delivery

Versatile reagent for standard and challenging cells. Fully compatible with suspension cells. Highly efficient and safe.

 

Sufficient for the average number of * transfections in a 24-well format.

Choose:

Viromer® CRISPR – powerful transfection for genome-editing

Viromer® CRISPR is optimized for in vitro delivery of Cas9 Ribonucleoprotein (RNP), i.e. pre-complex of the Cas9 endonuclease with one specific guide RNA. Compared to DNA-based approaches, RNP delivery enables faster and higher genome editing, and it offers a powerful alternative for difficult cells.

 

Which Viromer® for CRISPR/Cas9 experiments?

If you rather choose to transfect plasmid DNA or mRNA encoding Cas9 protein, we recommend using the Viromer® RED.
For transfection of guide RNA into Cas9-stable cells, please try Viromer® BLUE or Viromer® GREEN.

Success of RNP delivery and subsequent genome editing effect will depend on transfection efficiency. It will vary with cell types and can require optimization. To check Viromer® transfection efficiency by cell type, please consult our cell database.


Applications

  • in vitro delivery of Cas9 Ribonucleoprotein (RNP)
  • cancer research
  • stem cell research
  • cell signaling

Features and Benefits

  • High transfection efficiency due to an active escape of Viromer® complexes from the endosome.
  • Great safety because Viromer® complexes are non-charged, gentle on cells and compatible with serum and antibiotics.
  • Easy and fast transfection with consistent results ascribed to straightforward protocol including initial optimization.

Notes

  • Buffer CRISPR, pH 7.2 is supplied with the kit
  • for research use only
  • store dry at 2-8°C