Viromer® CRISPR is optimized for in vitro delivery of Cas9 Ribonucleoprotein (RNP), i.e. pre-complex of the Cas9 endonuclease with one specific guide RNA. Compared to DNA-based approaches, RNP delivery enables faster and higher genome editing, and it offers a powerful alternative for difficult cells.
Which Viromer® for CRISPR/Cas9 experiments?
If you rather choose to transfect plasmid DNA or mRNA encoding Cas9 protein, we recommend using the Viromer® RED.
For transfection of guide RNA into Cas9-stable cells, please try Viromer® BLUE or Viromer® GREEN.
Success of RNP delivery and subsequent genome editing effect will depend on transfection efficiency. It will vary with cell types and can require optimization. To check Viromer® transfection efficiency by cell type, please consult our cell database.