Viromer® GREEN

siRNA / miRNA transfection

Selected for fibroblasts, colon carcinoma or in THP-1 transfection.

Standard and other challenging cells might prefer BLUE.

Sufficient for the average number of * transfections in a 24-well format.

Choose:

Strong reagent for the THP-1 transfection with siRNA and miRNA

Viromer® GREEN works extremely efficient in the siRNA transfection of fibroblasts, colon carcinoma or in THP-1 transfection. This transfection reagent is especially developed for the binding of smaller oligonucleotides that are used in the RNAi technology like siRNA and the regulation of protein expresson via miRNA. It is based on a medium sized branched polymer that provides an active endosome escape due to it’s substitutions. With this technology it is even possible to go for really hard-to-transfect suspension cells, especially the use of siRNA in THP-1 transfection.


THP-1: transfection is difficult due to their non-adhesive character

Especially the THP-1 transfection, which became a common cell line in immunology research, is known to be very difficult. THP-1 are human monocytic cells that are non-adhesive, cultured in suspension and were originally derived from a patient with acute monocytic leukemia. These cells grow in RPMI medium with 10% FCS and 5% CO2 and due to their non-adhesive character it is a common knowledge, that there are difficulties in the attachment of the transfection complexes with the cells. Viromer® GREEN provides great efficiencies in THP-1 transfection with siRNA and also in other cells like primary human fibroblasts and lots of different cancer cell lines.


THP-1: transfection with miRNA / siRNA

Since miRNA / siRNA have the size of just a few nucleotides, it turns out to be much more easier to use these targets for THP-1 transfection than big plasmids. The efficiency in THP-1 transfection with Viromer® GREEN is up to 90% and provides you a safe and efficient way for a gene knockdown in these hard-to-transfect cells. Download the easy-to-use quick guide or the full transfection protocol here and prepare for your next THP-1 transfection experiment.

Knockdown of MyD88 and AP2 in THP-1 monocytes transfected with Viromer® GREENWesternblot of PKD2 knockdown in THP-1 monocytes transfected with Viromer® GREENShift in FACS analysis after transfection of Blockit red fluorescent oligo into THP-1 monocytes with Viromer® GREEN


THP-1: transfection of mRNA and plasmid DNA

The huge interest of many researchers for a protein overexpression of their target of interest in these special cells comes with a big problem. It’s becoming more and more public that these cells have a very high DNAse activity in their cytosol, so a THP-1 transfection with plasmid DNA is not working: the released DNA is digested by a DNAse. If you are looking for a protein overexpression, refer to a THP-1 transfection with mRNA. Have a look at our ready-to-use Viromer® RED pDNA- / mRNA-GFP controls and their great efficiency in THP-1 transfection with mRNA. Use the preformulated GFP-control for a first comparison of your cells between plasmid DNA and mRNA transfection and follow with a test of pure Viromer® RED and your special target.

Overexpression of GFP in THP-1 monocytes transfected with Viromer® RED GFP-mRNA positive control

 


Applications

  • RNA interference with siRNA
  • regulation of protein expression via miRNA
  • cancer research
  • stem cell research
  • cell signaling

Features and Benefits

  • High transfection efficiency due to an active escape of Viromer® complexes from the endosome.
  • Great safety because Viromer complexes are non-charged, gentle on cells and compatible with serum and antibiotics.
  • Easy and fast transfection with consistent results ascribed to straightforward protocol including initial optimization.

Notes

  • Buffer GREEN, pH 7.2 is supplied with the kit
  • for research use only
  • store dry at 2-8°C

Publications for miRNA / siRNA transfection with Viromer® GREEN

Enhancer of rudimentary homologue interacts with scaffold attachment factor B at the nuclear matrix to regulate SR protein phosphorylation.

Drakouli et. al., FEBS J., 2017

Targeting Deficiencies in the TLR5 Mediated Vaginal Response to Treat Female Recurrent Urinary Tract Infection.
Ali et. al., Sci Rep., 2017
HCMV activation of ERK-MAPK drives a multi-factorial response promoting the survival of infected myeloid progenitors.

Kew et. al., J Mol Biochem., 2017

Macrophage inducible C-type lectin (Mincle) recognizes glycosylated surface (S)-layer of the periodontal pathogen Tannerella forsythia.

Chinthamani et. al., PLoS ONE, 2017

Differential IL-1β secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability.

Hadadi et.al., Sci Rep.,  2016

Loss of the Tumor Suppressor NKX3.1 in Prostate Cancer Cells is Induced by Prostatitis Related Mitogens

Decker et.al., J Clin Onc Exp Onc, 2016

Schnitzer - 2016
Implications of a genetically determined nitric oxide deficit for endothelial cell-leukocyte interaction and cardiovascular disease

Kadiyska - 2016

Pathways of retinoid synthesis in mouse macrophages and bone marrow cells.

Niu et. al., J Leukoc Biol, 2016

Pattern recognition receptor mediated downregulation of microRNA-650 fine-tunes MxA expression in dendritic cells infected with Influenza A virus

Pichulik et.al., Eur J Immunol, 2015

Time-resolved quantitative proteomics implicates the core snRNP protein SmB together with SMN in neural trafficking.

Prescott et. al., J Cell Sci., 2014