siRNA / miRNA transfection
Selected for fibroblasts, colon carcinoma or in THP-1 transfection.
Standard and other challenging cells might prefer BLUE.
Viromer® GREEN works extremely efficient in the siRNA transfection of fibroblasts, colon carcinoma or in THP-1 transfection. This transfection reagent is especially developed for the binding of smaller oligonucleotides that are used in the RNAi technology like siRNA and the regulation of protein expresson via miRNA. It is based on a medium sized branched polymer that provides an active endosome escape due to it’s substitutions. With this technology it is even possible to go for really hard-to-transfect suspension cells, especially the use of siRNA in THP-1 transfection.
Especially the THP-1 transfection, which became a common cell line in immunology research, is known to be very difficult. THP-1 are human monocytic cells that are non-adhesive, cultured in suspension and were originally derived from a patient with acute monocytic leukemia. These cells grow in RPMI medium with 10% FCS and 5% CO2 and due to their non-adhesive character it is a common knowledge, that there are difficulties in the attachment of the transfection complexes with the cells. Viromer® GREEN provides great efficiencies in THP-1 transfection with siRNA and also in other cells like primary human fibroblasts and lots of different cancer cell lines.
Since miRNA / siRNA have the size of just a few nucleotides, it turns out to be much more easier to use these targets for THP-1 transfection than big plasmids. The efficiency in THP-1 transfection with Viromer® GREEN is up to 90% and provides you a safe and efficient way for a gene knockdown in these hard-to-transfect cells. Download the easy-to-use quick guide or the full transfection protocol here and prepare for your next THP-1 transfection experiment.
The huge interest of many researchers for a protein overexpression of their target of interest in these special cells comes with a big problem. It’s becoming more and more public that these cells have a very high DNAse activity in their cytosol, so a THP-1 transfection with plasmid DNA is not working: the released DNA is digested by a DNAse. If you are looking for a protein overexpression, refer to a THP-1 transfection with mRNA. Have a look at our ready-to-use Viromer® RED pDNA- / mRNA-GFP controls and their great efficiency in THP-1 transfection with mRNA. Use the preformulated GFP-control for a first comparison of your cells between plasmid DNA and mRNA transfection and follow with a test of pure Viromer® RED and your special target.
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Kadiyska - 2016
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