Viromer® RED

mRNA / plasmid transfection

Versatile reagent for standard and challenging cells. Highly efficient and safe.

A range of cells prefers YELLOW. Fully compatible with the lyophilized Viromer®  ONE RED transfection platform.

Sufficient for the average number of * transfections in a 24-well format.


Unsurpassed mRNA and plasmid transfection reagent

Viromer® RED has been extensively optimized for binding of large oligonucleotides such as mRNA and plasmids. By using the innovative Viromer® technology excellent delivery of mRNA and plasmid transfection complexes into standard cells like HEK-293, HeLa and CHO as well as in challenging cells such as C2C12 myoblasts and primary cells of macrophages, keratinocytes and pancreatic tumor cells is achieved. Combined with an active endosome escape mechanism and a great safety researchers get unsurpassed mRNA and plasmid transfection efficacy. Very high plasmid transfection efficiency up to 80% can be achieved in primary human macrophages. Based on the fact that primary keratinocytes are predestinated to prevent uptake of any material an outcome of 65% plasmid transfection efficiency in these challenging cells by using Viromer® RED is a fantastic result. Please have a look to our extensive cell-data-base which includes all cell types where transfection using Viromer® results in an efficiency of mRNA/plasmid transfection up to 90%.

Efficient in mRNA and plasmid transfection of challenging cells

GFP overexpression in primary human monocytes transfected with Viromer® REDoverexpression of a HA-tagged protein in primary human macrophages transfected with Viromer® REDGFP overexpression in RAW 264.7 macrophages transfected with Viromer® RED

GFP overexpression in primary human keratinocytes transfected with Viromer® REDRFP overexpression in H9c2 cardiomyocytes transfected with Viromer® REDmCherry overexpression in primary human pancreatic tumor cells transfected with Viromer® RED


  • protein expression / overexpression
  • RNA interference with plasmids encoding for shRNA
  • reporter gene assays
  • cancer research
  • stem cell research
  • cell signaling

Features and Benefits

  • High transfection efficiency due to an active escape of Viromer® complexes from the endosome.
  • Great safety because Viromer complexes are non-charged, gentle on cells and compatible with serum and antibiotics.
  • Easy and fast transfection with consistent results ascribed to straightforward protocol including initial optimization.


  • Buffer RED, pH 6.0 is supplied with the kit
  • for research use only
  • store dry at 2-8°C

Publications for shRNA / mRNA / plasmid transfection with Viromer® RED

Uracil-DNA glycosylase UNG1 isoform variant supports class switch recombination and repairs nuclear genomic uracil.

Sarno et. al., Nucleic Acids Res., 2019

Transcriptional cofactor Vgll2 is required for functional adaptations of skeletal muscle induced by chronic overload.

Honda et. al., J Cell Physiol., 2019

Severe fever with thrombocytopenia syndrome phlebovirus non-structural protein activates TPL2 signalling pathway for viral immunopathogenesis.

Choi et. al., Nat Microbiol., 2019

Characterization of Cells Interactions with Patterned Azopolymer-Based Materials using SEM, AFM and Video Microscopy

Barillé et. al., The Open Biomedical Engineering Journal, 2018

Impact of Variant Notch Ligandson Chronic Liver Diseases

Dewidar, 2018

Exploitation of Synthetic mRNA To Drive Immune Effector Cell Recruitment and Functional Reprogramming In Vivo.

Xu et. al., J Immunol., 2019

Muscle A-kinase-anchoring protein-β-bound calcineurin toggles active and repressive transcriptional complexes of myocyte enhancer factor 2D.

Li et. al., J Biol Chem., 2019

Early growth response-1 regulates acetylcholinesterase and its relation with the course of Alzheimer’s disease.

Hu et. al., Brain Pathol., 2019

Induction of immunosuppressive functions and NF-κB by FLIP in monocytes.

Fiore et. al., Nat Commun., 2018

Mechanosensorische Prozesse zur Erkennung und Prozessierung zyklischer Dehnungwährend der epithelialen Differenzierung

Noethel, 2018

Organellar Proteomics and Phospho-Proteomics Reveal Subcellular Reorganization in Diet-Induced Hepatic Steatosis

Krahmer et. al., Developmental Cell, 2018

JAG2 signaling induces differentiation of CD14+ monocytes into Langerhans cell histiocytosis‐like cells

Schwentner et. al., J Leukoc Biol. 2019

Engineered mRNA-expressed antibodies prevent respiratory syncytial virus infection

Tiwari et. al., Nature Communications, 2018

Effectiveness of EGFR/HER2-targeted drugsis influenced by the downstreaminteraction shifts of PTPIP51 in HER2-amplified breast cancer cells

Dietel et. al., Oncogenesis, 2018

Composition For Targeted Delivery Of Nucleic Acid-Based Therapeutics

Paukshto et. al., United States Patent Application 20180214575, 2018

JNK regulates muscle remodeling via myostatin/SMAD inhibition.

Lessard et. al., Nat Commun. 2018

Synthetic mRNA expressed Cas13a mitigates RNA virus infections

Bawage et. al. , BioRxiv, 2018

Epigenome-wide DNA methylation profiling in Progressive Supranuclear Palsy reveals major changes at DLX1.

Weber et. al., Nat Commun., 2018

Transition of responsive mechanosensitive elements from focal adhesions to adherens junctions upon epithelial differentiation.

Noethel et. al., Mol Biol Cell., 2018

GATA3 as a master regulator and therapeutic target in ovarian high-grade serous carcinoma stem cells.

Chen et. al., Int J Cancer., 2018

Immunostimulatory guide RNAs mediate potent antiviral response

Cai et. al., BioRxiv, 2018

Apoptotic Cancer Cells Suppress 5-Lipoxygenase in Tumor-Associated Macrophages.

Ringleb et. al., J Immunol., 2018

CRISPR/Cas9 knockout of USP18 enhances type I IFN responsiveness and restricts HIV-1 infection in macrophages.

Taylor et. al., J Leukoc Biol., 2018

Unifying in vitro and in vivo IVT mRNA expression discrepancies in skeletal muscle via mechanotransduction.

Bhosle et. al., Biomaterials. 2018

Long 3’UTR of Nurr1 mRNAs is targeted by miRNAs in mesencephalic dopamine neurons.

Pereira et. al., PLoS One., 2017

Developmental YAPdeltaC determines adult pathology in a model of spinocerebellar ataxia type 1.

Fujita et. al., Nat Commun., 2017

The Oxidative State of Cysteine Thiol 144 Regulates the SIRT6 Glucose Homeostat

Long et. al., Sci Rep., 2017

E-cadherin integrates mechanotransduction and EGFR signaling to control junctional tissue polarization and tight junction positioning.

Rübsam et. al., Nat Commun. 2017

The scaffold protein Ajuba suppresses CdGAP activity in epithelia to maintain stable cell-cell contacts.

McCormack et. al., Sci Rep., 2017

Kirschman – 2017
Caffeine induces gastric acid secretion via bitter taste signaling in gastric parietal cells

Liszt et. al., Proc Natl Acad Sci U S A., 2017

The embryonic type of SPP1 transcriptional regulation is re-activated in glioblastoma.

Kijewska et. al., Oncotarget., 2017

Live cell imaging of mitochondria following targeted irradiation in situ reveals rapid and highly localized loss of membrane potential.

Walsh et. al., Sci Rep., 2017

Characterizing exogenous mRNA delivery, trafficking, cytoplasmic release and RNA-protein correlations at the level of single cells.

Kirschman, Nucleic Acids Res., 2017

Hmga2 translocation induced in skin tumorigenesis.

Li et. al., Oncotarget., 2017

IKKα controls ATG16L1 degradation to prevent ER stress during inflammation.

Diamanti et. al., J Exp Med., 2017

Single α-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites.

Muggiolu et. al., Sci Rep., 2017

The epidermal polarity protein Par3 is a non-cell autonomous suppressor of malignant melanoma.

Mescher et. al., J Exp Med.,  2017

The p7 viroporin of the hepatitis C virus contributes to liver inflammation by stimulating production of Interleukin-1 β.

Farag et. al., Biochim Biophys Acta., 2016

Defining functional interactions during biogenesis of epithelial junctions

Erasmus, Nat Commun. 2016

Lipocalin 2 from macrophages stimulated by tumor cell-derived sphingosine 1-phosphate promotes lymphangiogenesis and tumor metastasis.

Jung et. al., Sci Signal., 2016

Extensive nuclear sphere generation in the human Alzheimer’s brain

Kolbe et. al., NeuroBiolAging, 2016

Hepatic Fgf21 Expression Is Repressed after Simvastatin Treatment in Mice.

Ziros et. al., PLoS One., 2016

Systemic RNA delivery to dendritic cells exploits antiviral defence for cancer immunotherapy.

Kranz et. al., Nature. 2016

Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression.

Shen et. al., Am J Pathol., 2016

Tumor suppressor BTG1 promotes PRMT1-mediated ATF4 function in response to cellular stress.

Yuniati et. al., Oncotarget, 2016

Drug Delivery Using Novel Biological and Synthetic Materials

Ito et. al., Biomed Res Int, 2015

The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells

Rao, Hindawi Publishing Group, BioMed Research International, 2015