Viromer® RED

mRNA / plasmid transfection

Versatile reagent for standard and challenging cells. Highly efficient and safe.

A range of cells prefers YELLOW. Fully compatible with the lyophilized Viromer®  ONE RED transfection platform.

Sufficient for the average number of * transfections in a 24-well format.

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Unsurpassed mRNA and plasmid transfection reagent

Viromer® RED has been extensively optimized for binding of large oligonucleotides such as mRNA and plasmids. By using the innovative Viromer® technology excellent delivery of mRNA and plasmid transfection complexes into standard cells like HEK-293, HeLa and CHO as well as in challenging cells such as C2C12 myoblasts and primary cells of macrophages, keratinocytes and pancreatic tumor cells is achieved. Combined with an active endosome escape mechanism and a great safety researchers get unsurpassed mRNA and plasmid transfection efficacy. Very high plasmid transfection efficiency up to 80% can be achieved in primary human macrophages. Based on the fact that primary keratinocytes are predestinated to prevent uptake of any material an outcome of 65% plasmid transfection efficiency in these challenging cells by using Viromer® RED is a fantastic result. Please have a look to our extensive cell-data-base which includes all cell types where transfection using Viromer® results in an efficiency of mRNA/plasmid transfection up to 90%.


Efficient in mRNA and plasmid transfection of challenging cells

GFP overexpression in primary human monocytes transfected with Viromer® REDoverexpression of a HA-tagged protein in primary human macrophages transfected with Viromer® REDGFP overexpression in RAW 264.7 macrophages transfected with Viromer® RED

GFP overexpression in primary human keratinocytes transfected with Viromer® REDRFP overexpression in H9c2 cardiomyocytes transfected with Viromer® REDmCherry overexpression in primary human pancreatic tumor cells transfected with Viromer® RED


Applications

  • protein expression / overexpression
  • RNA interference with plasmids encoding for shRNA
  • reporter gene assays
  • cancer research
  • stem cell research
  • cell signaling

Features and Benefits

  • High transfection efficiency due to an active escape of Viromer® complexes from the endosome.
  • Great safety because Viromer complexes are non-charged, gentle on cells and compatible with serum and antibiotics.
  • Easy and fast transfection with consistent results ascribed to straightforward protocol including initial optimization.

Notes

  • Buffer RED, pH 6.0 is supplied with the kit
  • for research use only
  • store dry at 2-8°C

Publications for mRNA / plasmid transfection with Viromer® RED

The scaffold protein Ajuba suppresses CdGAP activity in epithelia to maintain stable cell-cell contacts.

McCormack et. al., Sci Rep., 2017

CHARACTERIZATION OF IN VITRO TRANSCRIBED MRNA FOR OPTIMAL EXPRESSION IN THERAPEUTIC APPLICATIONS
Kirschman – 2017
Caffeine induces gastric acid secretion via bitter taste signaling in gastric parietal cells

Liszt et. al., Proc Natl Acad Sci U S A., 2017

The embryonic type of SPP1 transcriptional regulation is re-activated in glioblastoma.

Kijewska et. al., Oncotarget., 2017

Live cell imaging of mitochondria following targeted irradiation in situ reveals rapid and highly localized loss of membrane potential.

Walsh et. al., Sci Rep., 2017

Characterizing exogenous mRNA delivery, trafficking, cytoplasmic release and RNA-protein correlations at the level of single cells.

Kirschman et.al., Nucleic Acids Res., 2017

Hmga2 translocation induced in skin tumorigenesis.

Li et. al., Oncotarget., 2017

IKKα controls ATG16L1 degradation to prevent ER stress during inflammation.

Diamanti et. al., J Exp Med., 2017

Single α-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites.

Muggiolu et. al., Sci Rep., 2017

The epidermal polarity protein Par3 is a non-cell autonomous suppressor of malignant melanoma.

Mescher et. al., J Exp Med.,  2017

The p7 viroporin of the hepatitis C virus contributes to liver inflammation by stimulating production of Interleukin-1 β.

Farag et. al., Biochim Biophys Acta., 2016

Defining functional interactions during biogenesis of epithelial junctions

Erasmus et.al., Nat Commun. 2016

Extensive nuclear sphere generation in the human Alzheimer’s brain

Kolbe et. al., NeuroBiolAging, 2016

Hepatic Fgf21 Expression Is Repressed after Simvastatin Treatment in Mice.

Ziros et. al., PLoS One., 2016

Systemic RNA delivery to dendritic cells exploits antiviral defence for cancer immunotherapy.

Kranz et. al., Nature. 2016

Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression.

Shen et. al., Am J Pathol., 2016

Tumor suppressor BTG1 promotes PRMT1-mediated ATF4 function in response to cellular stress.

Yuniati et. al., Oncotarget, 2016

Drug Delivery Using Novel Biological and Synthetic Materials

Ito et. al., Biomed Res Int, 2015

The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells

Rao et.al., Hindawi Publishing Group, BioMed Research International, 2015