Viromer® in neurobiology research

No doubt, that neurobiology is a major field of current medical research. Many labs are working hard to define the causes of mental diseases like psychotic disorders, Parkinson’s syndrome, Alzheimer’s disease, or addiction. More fundamentally, there is still a lack of knowledge on physiological aspects of sensory or motor systems, mechanisms involved in the development and functioning of consciousness, perception, memory, effects of aging or injuries, and neuroplasticity. Cancers affecting the nervous system are also intensively studied.

One challenge for neurobiologists is to manipulate cell or animal models in the lab, for which transfection has notably a strong limitation. Primary neurons and neural stem cells are for instance not easily amenable to transfection mediated by chemical reagents because of reduced endocytosis and nuclear entry. Electroporation or viral transduction remain the main used method for these cells.

Microscopic observations of SH-SY5Y neuroblastoma transfected with pEGFPN2 and pNFH-GFP by using Viromer® RED. Data from F. Letournel, CHU Angers, France

siRNA-mediated gene silencing

Viromer® BLUE and GREEN are powerful tools for siRNA-mediated strong knockdowns in a broad range of nerve cells

  • immune cells: BV2 and primary microglias
  • primary astrocytes or Schwann cells
  • cancer cell lines: SH-SY5Y, Neuro2A, mouse pheochromocytoma, T96G and Tu-132 and a list of more than 10 glioblastoma cells

protein expression

Transfection of pDNA with Viromer® RED achieves very good levels of protein expression in SH-SY5Y, Neuro2A, primary oligodendrocytes, astrocytes and some immune cells like BV2. We have shown higher levels of protein expression SH-SY5Y and Neuro2A.

Comparison of GFP expression in rat brain astrocytes (DI TNC1) cells after plasmid (pMaxGFP) transfection with Viromer® RED, Lipofectamine® 3000 and Lipofectamine® LTX.

Microscopic observations of rat primary oligodendrocytes transfected with pMAX-GFP after 5 days of differentiation (in vitro) by using Viromer® RED.

The Viromer® technology will not solve the problems in transfection of primary neurons, even if some data show promising results (e.g. transfection of mRNA), but it opens great perspectives for that field, especially when you have the ability to sort the positive cells.

mRNA versus pDNA

As detailed at the Viromer® in mRNA transfection page, mRNA rather than plasmid transfection can solve difficulties encountered to overexpress proteins in some specific cases. Take some time and go through the following neural cell database below und see, how Viromer can support your research.

Colors

Viromer® BLUE | Viromer® GREEN | Viromer® RED | Viromer® YELLOW

transfection efficiency

< 30% | 30-50% | 50-80% | > 80%

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Type siRNA mRNA pDNA Data
A-172       
Astrocytes, primary mouse      See data
BV-2            See data Publications
C17.2     
C6     See data Publication
DI TNC 1      See data
Dorsal root ganglion cells, primary human      Publication
F11 (HB-11761)   
GB3     See data
GH3      See data
hCMEC/D3  
LN-229        See data Publication
LN-308     Publication
LN18      Publication
Microglia, primary murine      See data Publications
N9      See data
NCH149          See data
NCH82          See data
Neuro-2A                       See data
Neurons, primary murine      See data Publication