Viromer® RED and Viromer® YELLOW have been designed to work equally strong with DNA and mRNA, even enabling their co-transfection. Based on internal tests and users’ feedbacks, we have demonstrated a clear advantage of mRNA transfection in general, but more interestingly for some specific cells known as “resistant” to plasmid transfection:
- cells with low division rate, e.g. primary neurons, differentiated skeletal muscle cells, and
- cells with cytosolic defense mechanisms against foreign DNA (innate immunity), e.g. AIM2-Interleukin or cGAS-Interferon enzymatic cascades of macrophages and monocytes.
We have successfully transfected mRNA into several model cell lines (CHO, HEK, HeLa, HepG2, C2C12, MDCK, HUVEC), but also in some specific cancer cells (A549, H322, H358, Neuro2A, SH-SH5Y), primary neurons, and immune MPS cells (primary macrophages, monocytes and dendritic cells, THP-1 monocytes, J774 macrophages). For more details, please go to the cell database.