Viromer® transfection reagent is a strong tool for topical in vivo applications in animal models.
15μg of complexed mRNA were injected into the mouse duodenum, luciferase was monitored after 6h. For imaging purposes, the abdomen was opened and the intestine exposed.
Expression of luciferase was detected in doudenum and small intestine.
2μg of complexed mRNA were injected intradermally close to the shoulder, luciferase was monitored after 6h.
Expression of luciferase is clearly visible as a localized signal.