Human obesity is one of the most important diseases of the western world and a leading cause of death worldwide. It increases the likelihood for the manifestation of cardiovascular diseases, type 2 diabetes, different kinds of cancer, depression and asthma. Since obesity has been found to be one of the most important public health problems of the 21st century, research started to focus more and more on the metabolic system and the defective consequences for the human body. What is the cause of obesity? How can we prevent it? Which cellular mechanisms are involved? Can we treat the cells with medication to minimize or stop the production of fatty acids. What ways do we have to force these cells to lose stored fatty acids?
The morbidity due to the increased number of fat cells includes diabetes, cancer, cardiovascular disease and non-alcohol associated liver diseases. The in vitro transfection of these related cells is an unevitable necessity in research to understand the molecular mechanisms behind, on the way to address the fight against obesity related diseases. Since Viromer® transfection reagents are polymer based and lipid-free, there is no interaction with the cell metabolism and in particular with the lipid metabolism, which makes them the perfect transfection reagent for adipocytes and liver cells in comparison to lipid based reagents. The Viromer® reagents provide excellent results in adipocytes, hepatocytes, fibroblasts, kidney cells and pancreatic beta cells – as well as for knockdown studies with siRNA or overexpression experiments with pDNA or mRNA.