primary human macrophages
transfected with Viromer® BLUE
- freshly isolated from buffy coat PBMCs
- HTS-setup, transfected in a 384-well plate
- siRNA targeting EEA1
primary human macrophages
transfected with Viromer® RED
-
primary human monocyte derived macrophages
-
6-well setup
-
Only Viromer® RED shows reproducible and satisfied overexpression of the target protein.
primary human mesenchymal stem cells
transfected with Viromer® BLUE
- nearly 4-fold target gene knock-down of protein x
primary human fibroblasts
transfected with Viromer® GREEN
-
siGlo oligo was co-transfected with the siRNA of interest
-
pictures were taken at 2.5 x magnification
primary human keratinocytes
transfected with Viromer® BLACK
- 85% knockdown
primary human pancreatic tumor cells
transfected with Viromer® RED
- derived directly from patient tumor
- 500ng of an mCherry plasmid
primary neonatal rat ventricular cardiomyocytes
transfected with Viromer® YELLOW
- 1 x 105 cells/well
-
24-well culture plate
-
after 24-48 h in culture, cells were transfected with 0,5μg of a plasmid expressing GFP
Primary human monocytes
transfected with Viromer® RED
- CD14 purified monocytes from human PBMCs (Buffy Coat)
- 40.000 cells / 96-well
- treatment with various doses of GFP-mRNA
- GFP detection via MACSQuant®
90% positive transfected cells, no visible toxicity
primary keratinocytes
transfected with Viromer® RED
- seed 200.000 cells / 6 well
- transfection efficiency evaluated 24h post transfection (FACS)
