primary human macrophages

transfected with Viromer® RED

• primary human monocyte derived macrophages
• 6-well setup
• Only Viromer® RED shows reproducible and satisfied overexpression of the target protein.

primary human mesenchymal stem cells

transfected with Viromer® BLUE

• nearly 4-fold target gene knock-down of protein x

primary human fibroblasts

transfected with Viromer® GREEN

• siGlo oligo was co-transfected with the siRNA of interest
• pictures were taken at 2.5 x magnification

primary human keratinocytes

transfected with Viromer® BLACK

• 85% knockdown

primary human pancreatic tumor cells

transfected with Viromer® RED

• derived directly from patient tumor
• 500ng of an mCherry plasmid

primary neonatal rat ventricular cardiomyocytes

transfected with Viromer® YELLOW

• 1 x 105 cells/well
• 24-well culture plate
• after 24-48 h in culture, cells were transfected with 0,5μg of a plasmid expressing GFP

Primary human monocytes

transfected with Viromer® RED

• CD14 purified monocytes from human PBMCs (Buffy Coat)
• 40.000 cells / 96-well
• treatment with various doses of GFP-mRNA
• GFP detection via MACSQuant®

90% positive transfected cells, no visible toxicity

primary keratinocytes

transfected with Viromer® RED

• seed 200.000 cells / 6 well
• transfection efficiency evaluated 24h post transfection (FACS)