Nowadays the common and most cited delivery methods for the CRISPR / Cas9 technology are electroporation, viral transduction, or lipofection. But major drawbacks are still not overpassed since impacts on cell physiology or viability are often reported for many cell types. Electroporation is also known to be cost-effective and viral transduction requires expertise and tedious protocols.
By screening more than 10.000 different polymer compositions and formulations, VIROMER® CRISPR offers now a chemical transfection tool. It was developed and especially optimized for a reliable delivery of the ribonucleoprotein (RNP) complex of Cas9 endonuclease and target guide RNA. Maximized transfection efficiencies in different cell types together with low cell toxicity are leading to a high Cas9-mediated genome editing efficiency with less off-target effects.
A clear advantage of Cas9 RNP delivery is a faster and safer genome editing process. Once delivered into the cytosol there is an immediate usage of the active endonuclease. In comparison using plasmid vectors is the easiest method but it slows down the process due to transcriptional and translational steps and it doesn’t work for hard-to-transfect cells. VIROMER® CRISPR is now available to give safety to a crucial part of the CRISPR / Cas9 genome editing workflow, which is the delivery.